蛋白質(zhì)和多肽電泳根據(jù)其等電點(diǎn)、電荷態(tài)、尺寸或這些屬性的組合進(jìn)行分離。毛細(xì)管電泳 (CE) 通常應(yīng)用于基于電荷態(tài)的完整蛋白質(zhì)分離以及糖基化分析。毛細(xì)管電泳還能同質(zhì)譜儀聯(lián)用，常用作液質(zhì)聯(lián)用的正交技術(shù)。簡(jiǎn)單的電泳技術(shù)（如 SDS-PAGE）通常用于蛋白質(zhì)穩(wěn)定性測(cè)試、質(zhì)量控制、對(duì)蛋白質(zhì)表達(dá)進(jìn)行線上監(jiān)控以及蛋白質(zhì)純化的優(yōu)化和監(jiān)控。

以下應(yīng)用簡(jiǎn)報(bào)對(duì)使用 Agilent 7100 毛細(xì)管電泳系統(tǒng)進(jìn)行蛋白質(zhì)表征以及同飛行時(shí)間質(zhì)譜聯(lián)用進(jìn)行蛋白質(zhì)修飾分析進(jìn)行了介紹。安捷倫提議將 2200 TapeStation 和 2100 生物分析儀用作 SDS-PAGE 凝膠的替代平臺(tái)，用于監(jiān)測(cè)整個(gè)流程開(kāi)發(fā)過(guò)程中的蛋白質(zhì)命運(yùn)。了解有關(guān)安捷倫 CE/MS 解決方案的更多信息。

• 使用毛細(xì)管電泳和質(zhì)譜聯(lián)用技術(shù)表征小分子量免疫交聯(lián)物 (< 40 kDa) (Characterizationof Small Immunoconjugates (< 40 kDa) UsingCapillary Electrophoresis – Mass Spectrometry)5991-4433EN• 將新型發(fā)光二極管誘導(dǎo)熒光檢測(cè)器與 Agilent7100 毛細(xì)管電泳系統(tǒng)聯(lián)用進(jìn)行雜質(zhì)檢測(cè)(Impurity Detection with a New Light EmittingDiode Induced Fluorescence Detector Coupledto the Agilent 7100 Capillary ElectrophoresisSystem)5991-4043EN• 用于單克隆抗體制備過(guò)程中基因組 DNA去除和監(jiān)測(cè)的安捷倫解決方案 (AgilentSolutions for Removal and Monitoring ofGenomic DNA from Monoclonal AntibodyPreparations)5991-3527EN• CE/MS 與 LC/MS 的協(xié)同作用 — 肽圖分析的互補(bǔ)解決方案 (CE/MS and LC/MS Synergy –Complementary Solutions for Peptide Mapping)5991-2583EN

• 通過(guò)氟碳涂層毛細(xì)管的毛細(xì)管等電聚焦進(jìn)行單克隆抗體電荷異質(zhì)性分析 (MonoclonalAntibody Charge Heterogeneity Analysis byCapillary Isoelectric Focusing on FluorocarbonCoated Capillaries)5991-2885EN

• 使用 CE/MS/MS 作為正交技術(shù)實(shí)現(xiàn)靈敏且易于操作的復(fù)雜基質(zhì)中肽定量分析 (CE/MS/MS as an Orthogonal Technique for Sensitiveand Easy Quantification of Peptides in ComplexMatrixes)5991-2729EN• 使用毛細(xì)管電泳及四極桿飛行時(shí)間質(zhì)譜儀對(duì)單克隆抗體的氧化位點(diǎn)進(jìn)行鑒定及定量(Identification and Quantification of OxidationSites on Monoclonal Antibodies Using CapillaryElectrophoresis and Quadrupole Time-of-FlightMass Spectrometry)5991-2728EN• 單克隆抗體 N-糖鏈的毛細(xì)管電泳和質(zhì)譜分析5991-1020CHCN• 通過(guò) Agilent 7100 毛細(xì)管電泳系統(tǒng)的毛細(xì)管等電聚焦進(jìn)行單克隆抗體電荷異質(zhì)性分析5991-1142CHCN

• 采用 Agilent 7100 CE 系統(tǒng)通過(guò)毛細(xì)管凝膠電泳和 UV 檢測(cè)進(jìn)行蛋白質(zhì)表征以測(cè)定商品化凝膠的性能 (Performance of commerciallyavailable gels for protein characterization bycapillary gel electrophoresis with UV detectionon the Agilent 7100 CE System)5990-7976EN• 采用 Agilent 2100 生物分析儀在純化期間監(jiān)測(cè)蛋白質(zhì)命運(yùn) (Monitoring Protein Fate duringPurification with the Agilent 2100 Bioanalyzer)5990-6153EN• 通過(guò)毛細(xì)管電泳和 Q-TOF 質(zhì)譜法對(duì)抗體進(jìn)行糖肽分析 (Glycopeptide Analysis of Antibodiesby Capillary Electrophoresis and Q-TOF MassSpectrometry)5990-7138EN